Biosurfactant Production by Rhodococcus Erythropolis Sp. SB-1A Isolated from North Atlantic Ocean: Study on the Influence of Environmental Conditions

Authors

  • Tong Cao The Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, A1B 3X5, St. John’s, Canada
  • Baiyu Zhang The Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, A1B 3X5, St. John’s, Canada
  • Bing Chen The Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, A1B 3X5, St. John’s, Canada
  • Qinhong Cai The Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, A1B 3X5, St. John’s, Canada
  • He Zhang The Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, A1B 3X5, St. John’s, Canada
  • Zhiwen Zhu The Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, A1B 3X5, St. John’s, Canada
  • Kedong Zhang The Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, A1B 3X5, St. John’s, Canada

Abstract

Biosurfactant production by Rhodococcus erythropolis SB-1A was studied based on Atlas oil agar medium in a batch reactor. The strain was isolated from a water sample collected from Northern Atlantic Ocean. Several parameters including carbon source (n-hexadecane, 0.5, 2, 3.5 and 5 v/v%), nitrogen source (NH4NO3,0.4, 0.7, 1 and 1.3 g/L), pH (5,6, 7 and 8) and salinity (NaCl, 13, 26, 39 and 52 g/L) were analyzed to optimize cultural and environmental conditions for biosurfactant production. Surface active properties of biosurfactants in the cell-free broth were monitored periodically. Results showed that the crude biosurfactants in the media cultivated under experimentally defined conditions reduced surface tension by 40 dynes/cm within 10 hours of cultivation. The research outputs demonstrated the possibility of using biosurfactants produced by Rhodococcus erythropolis SB-1A for remedying hydrocarbon contamination in North Atlantic Canada and beyond.

 

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Published

2014-08-26

Issue

Proceedings of the International Conference on Marine and Freshwater Environments (iMFE 2014) - Our Water, Our Future

Section

Environmental and Marine Biotechnology (EMB)

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